• Western blot analysis of EpCAM. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:3000, RT, 1h.
  • Immunohistochemistry analysis of EpCAM. Sample: Human colon cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of EpCAM. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:200, RT, 1h.

Anti-EpCAM antibody [SM5087] (STJA0005087)

SKU:
STJA0005087

£170.50 - £233.50
Shipping:
Free Shipping
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-EpCAM is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM5087
Conjugation: Unconjugated
Isotype: IgG2ak
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (H) 1:1000-1:2000
IHC/IF (H) 1:2000-1:4000
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Epcam
Gene ID: 17075
Uniprot ID: EPCAM_MOUSE
Immunogen: Recombinant protein corresponding to Mouse EpCAM
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Protein Name Epithelial Cell Adhesion Molecule
Ep-Cam
Epithelial Glycoprotein 314
Egp314
Megp314
Protein 289a
Tumor-Associated Calcium Signal Transducer 1
Cd Antigen Cd326
Database Links Reactome: R-MMU-202733
Cellular Localisation Lateral Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Tight Junction
Colocalizes With Cldn7 At The Lateral Cell Membrane And Tight Junction
Alternative Antibody Names Anti-Epithelial Cell Adhesion Molecule antibody
Anti-Ep-Cam antibody
Anti-Epithelial Glycoprotein 314 antibody
Anti-Egp314 antibody
Anti-Megp314 antibody
Anti-Protein 289a antibody
Anti-Tumor-Associated Calcium Signal Transducer 1 antibody
Anti-Cd Antigen Cd326 antibody
Anti-Epcam antibody
Anti-Tacstd1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance