Western blot analysis of EpCAM. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-EpCAM antibody [SM5087] (STJA0005087)

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STJA0005087
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Short Description :Mouse monoclonal anti-EpCAM for use in WB, IHC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/IHC/IF
Host:Mouse
Reactivity:Human
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Monoclonal
Clone ID :SM5087
Isotype:IgG2ak
Conjugation:Unconjugated
Purification:Affinity purification
Dilution Range:WB (H) 1:1000-1:2000
IHC/IF (H) 1:2000-1:4000
Formulation:PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Storage Instruction:Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol:Epcam
Gene ID:17075
Uniprot ID:EPCAM_MOUSE
Immunogen:Recombinant protein corresponding to Mouse EpCAM
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Protein Name Epithelial Cell Adhesion Molecule
Ep-Cam
Epithelial Glycoprotein 314
Egp314
Megp314
Protein 289a
Tumor-Associated Calcium Signal Transducer 1
Cd Antigen Cd326
Database Links Reactome: R-MMU-202733
Cellular Localisation Lateral Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Tight Junction
Colocalizes With Cldn7 At The Lateral Cell Membrane And Tight Junction
Alternative Antibody Names Anti-Epithelial Cell Adhesion Molecule antibody
Anti-Ep-Cam antibody
Anti-Epithelial Glycoprotein 314 antibody
Anti-Egp314 antibody
Anti-Megp314 antibody
Anti-Protein 289a antibody
Anti-Tumor-Associated Calcium Signal Transducer 1 antibody
Anti-Cd Antigen Cd326 antibody
Anti-Epcam antibody
Anti-Tacstd1 antibody

Information sourced from Uniprot.org

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