| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ENPP7 |
| Gene ID: | 339221 |
| Uniprot ID: | ENPP7_HUMAN |
| Immunogen Region: | 250-350 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human ENPP7 (Q6UWV6). |
| Immunogen Sequence: | TTVDKRAGDLVEFHKFPNFT FRDIEFELLDYGPNGMLLPK EGRLEKVYDALKDAHPKLHV YKKEAFPEAFHYANNPRVTP LLMYSDLGYVIHGRINVQFN N |
| Tissue Specificity | Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon. |
| Post Translational Modifications | N-glycosylated.required for activity and transport to the plasma membrane. |
| Function | Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract. Has also phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation. Does not have nucleotide pyrophosphatase activity. May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen. |
| Protein Name | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 7 E-Npp 7 Npp-7 Alkaline Sphingomyelin Phosphodiesterase Intestinal Alkaline Sphingomyelinase Alk-Smase |
| Database Links | Reactome: R-HSA-9840310 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein The Catalytic Domain Is Released Into The Extracellular Medium When Cells Are Treated With Trypsin Localized At The Surface Of The Microvillar Membrane In Small Intestine Enterocytes And In Endosome-Like Structures Situated Beneath The Microvillar Membrane And In Golgi Complex |
| Alternative Antibody Names | Anti-Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 7 antibody Anti-E-Npp 7 antibody Anti-Npp-7 antibody Anti-Alkaline Sphingomyelin Phosphodiesterase antibody Anti-Intestinal Alkaline Sphingomyelinase antibody Anti-Alk-Smase antibody Anti-ENPP7 antibody Anti-UNQ3077 antibody Anti-PRO9912 antibody |
Information sourced from Uniprot.org