• Western blot analysis of extracts of mouse kidney, using KAT9/KAT9/Elp3 antibody (STJ111919) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
  • Immunohistochemistry analysis of paraffin-embedded mouse cerebellum using KAT9/KAT9/Elp3 antibody (STJ111919) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat cerebellum using KAT9/KAT9/Elp3 antibody (STJ111919) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat cerebellum using KAT9/KAT9/Elp3 antibody (STJ111919) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse cerebellum using KAT9/KAT9/Elp3 antibody (STJ111919) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of Mouse kidney, using KAT9/KAT9/Elp3 antibody (STJ111919) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-ELP3 antibody (1-200) (STJ111919)

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STJ111919

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ELP3 (1-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELP3
Gene ID: 55140
Uniprot ID: ELP3_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KAT9/Elp3 (NP_060561.3).
Immunogen Sequence: MRQKRKGDLSPAELMMLTIG DVIKQLIEAHEQGKDIDLNK VKTKTAAKYGLSAQPRLVDI IAAVPPQYRKVLMPKLKAKP IRTASGIAVVAVMCKPHRCP HISFTGNICVYCPGGPDSDF EYSTQSYTGYEPTSMRAIRA RYDPFLQTRHRIEQLKQLGH SVDKVEFIVMGGTFMALPEE YRDYFIRNLHDALSGHTSNN
Tissue Specificity Expressed in the cerebellum and spinal motor neurons.
Post Translational Modifications Tyrosine-phosphorylated.phosphorylation on Tyr-202 does not affect elongator complex integrity or ELP3 protein stability. Also serine/threonine-phosphorylated.
Function Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins.such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex.
Protein Name Elongator Complex Protein 3
Help3
Trna Uridine(34 Acetyltransferase
Database Links Reactome: R-HSA-3214847
Cellular Localisation Cytoplasm
Nucleus
Isoform 1: Nucleus
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Elongator Complex Protein 3 antibody
Anti-Help3 antibody
Anti-Trna Uridine(34 Acetyltransferase antibody
Anti-ELP3 antibody

Information sourced from Uniprot.org

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