CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ HepG5 with 1 Mu g ESE1 (STJ11102304) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of ESE1 in representative gene loci (MARK4) , as shown in figure.

Anti-ELF3 antibody (1-371) [S4MR] (STJ11102304)

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STJ11102304
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Host: Rabbit
Applications: WB/ELISA/ChIP/CUT&Tag
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
ChIP:5 Mu g antibody for 10 Mu g-15 Mu g of Chromatin
CUT&Tag: 10⁵ cells/1 Mu g
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELF3
Gene ID: 1999
Uniprot ID: ELF3_HUMAN
Immunogen Region: 1-371
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-371 of human ESE1/ELF3 (P78545).
Immunogen Sequence: MAATCEISNIFSNYFSAMYS SEDSTLASVPPAATFGADDL VLTLSNPQMSLEGTEKASWL GEQPQFWSKTQVLDWISYQV EKNKYDASAIDFSRCDMDGA TLCNCALEELRLVFGPLGDQ LHAQLRDLTSSSSDELSWII ELLEKDGMAFQEALDPGPFD QGSPFAQELLDDGQQASPYH PGSCGAGAPSPGSSDVSTAG TGASRSSHSSDSGGSDVDLD PTDGKLFPSDGFRDCKKGD
Tissue Specificity Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin.
Function Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGAAT. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.
Protein Name Ets-Related Transcription Factor Elf-3
E74-Like Factor 3
Epithelial-Restricted With Serine Box
Epithelium-Restricted Ets Protein Esx
Epithelium-Specific Ets Transcription Factor 1
Ese-1
Database Links Reactome: R-HSA-1912408
Cellular Localisation Cytoplasm
Nucleus
Localizes To The Cytoplasm Where It Has Been Shown To Transform Mcf-12a Mammary Epithelial Cells Via A Novel Cytoplasmic Mechanism
Also Transiently Expressed And Localized To The Nucleus Where It Induces Apoptosis In Non-Transformed Breast Epithelial Cells Mcf-10a And Mcf-12a Via A Transcription-Dependent Mechanism
Alternative Antibody Names Anti-Ets-Related Transcription Factor Elf-3 antibody
Anti-E74-Like Factor 3 antibody
Anti-Epithelial-Restricted With Serine Box antibody
Anti-Epithelium-Restricted Ets Protein Esx antibody
Anti-Epithelium-Specific Ets Transcription Factor 1 antibody
Anti-Ese-1 antibody
Anti-ELF3 antibody
Anti-ERT antibody
Anti-ESX antibody
Anti-JEN antibody

Information sourced from Uniprot.org

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