• Western blot analysis of extracts of 293T cells, using EGF antibody (STJ114782) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EGF antibody (23-250) (STJ114782)

SKU:
STJ114782

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EGF (23-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EGF
Gene ID: 1950
Uniprot ID: EGF_HUMAN
Immunogen Region: 23-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-250 of human EGF (NP_001954.2).
Immunogen Sequence: PQHWSCPEGTLAGNGNSTCV GPAPFLIFSHGNSIFRIDTE GTNYEQLVVDAGVSVIMDFH YNEKRIYWVDLERQLLQRVF LNGSRQERVCNIEKNVSGMA INWINEEVIWSNQQEGIITV TDMKGNNSHILLSALKYPAN VAVDPVERFIFWSSEVAGSL YRADLDGVGVKALLETSEKI TAVSLDVLDKRLFWIQYNRE GSNSLICSCDYDGGSVHISK HPTQHNLF
Tissue Specificity Expressed in kidney, salivary gland, cerebrum and prostate.
Post Translational Modifications O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.
Protein Name Pro-Epidermal Growth Factor
Egf Cleaved Into - Epidermal Growth Factor
Urogastrone
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1227986
Reactome: R-HSA-1236382
Reactome: R-HSA-1236394
Reactome: R-HSA-1250196
Reactome: R-HSA-1251932
Reactome: R-HSA-1257604
Reactome: R-HSA-177929
Reactome: R-HSA-179812
Reactome: R-HSA-180292
Reactome: R-HSA-180336
Reactome: R-HSA-182971
Reactome: R-HSA-1963640
Reactome: R-HSA-1963642
Reactome: R-HSA-212718
Reactome: R-HSA-2219530
Reactome: R-HSA-5637810
Reactome: R-HSA-5638303
Reactome: R-HSA-5673001
Reactome: R-HSA-6785631
Reactome: R-HSA-6811558
Reactome: R-HSA-8847993
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8863795
Reactome: R-HSA-9009391
Reactome: R-HSA-9013507
Reactome: R-HSA-9634638
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Pro-Epidermal Growth Factor antibody
Anti-Egf Cleaved Into - Epidermal Growth Factor antibody
Anti-Urogastrone antibody
Anti-EGF antibody

Information sourced from Uniprot.org

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