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Primary Antibodies
Polyclonal Antibodies
Anti-EGF antibody (23-250) (STJ114782)

Western blot analysis of extracts of 293T cells, using EGF antibody (STJ114782) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EGF antibody (23-250) (STJ114782)

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STJ114782

£169.50 - £449.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	WB
Reactivity:	Human
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-EGF (23-250) is suitable for use in Western Blot research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	EGF
Gene ID:	1950
Uniprot ID:	EGF_HUMAN
Immunogen Region:	23-250
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 23-250 of human EGF (NP_001954.2).
Immunogen Sequence:	PQHWSCPEGTLAGNGNSTCV GPAPFLIFSHGNSIFRIDTE GTNYEQLVVDAGVSVIMDFH YNEKRIYWVDLERQLLQRVF LNGSRQERVCNIEKNVSGMA INWINEEVIWSNQQEGIITV TDMKGNNSHILLSALKYPAN VAVDPVERFIFWSSEVAGSL YRADLDGVGVKALLETSEKI TAVSLDVLDKRLFWIQYNRE GSNSLICSCDYDGGSVHISK HPTQHNLF

Tissue Specificity	Expressed in kidney, salivary gland, cerebrum and prostate.
Post Translational Modifications	O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
Function	EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.
Protein Name	Pro-Epidermal Growth Factor Egf Cleaved Into - Epidermal Growth Factor Urogastrone
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-1227986 Reactome: R-HSA-1236382 Reactome: R-HSA-1236394 Reactome: R-HSA-1250196 Reactome: R-HSA-1251932 Reactome: R-HSA-1257604 Reactome: R-HSA-177929 Reactome: R-HSA-179812 Reactome: R-HSA-180292 Reactome: R-HSA-180336 Reactome: R-HSA-182971 Reactome: R-HSA-1963640 Reactome: R-HSA-1963642 Reactome: R-HSA-212718 Reactome: R-HSA-2219530 Reactome: R-HSA-5637810 Reactome: R-HSA-5638303 Reactome: R-HSA-5673001 Reactome: R-HSA-6785631 Reactome: R-HSA-6811558 Reactome: R-HSA-8847993 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 Reactome: R-HSA-8863795 Reactome: R-HSA-9009391 Reactome: R-HSA-9013507 Reactome: R-HSA-9634638 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686
Cellular Localisation	Membrane Single-Pass Type I Membrane Protein
Alternative Antibody Names	Anti-Pro-Epidermal Growth Factor antibody Anti-Egf Cleaved Into - Epidermal Growth Factor antibody Anti-Urogastrone antibody Anti-EGF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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