| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EEF2 |
| Gene ID: | 1938 |
| Uniprot ID: | EF2_HUMAN |
| Immunogen Region: | 1-290 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human eEF2 (NP_001952.1). |
| Immunogen Sequence: | MVNFTVDQIRAIMDKKANIR NMSVIAHVDHGKSTLTDSLV CKAGIIASARAGETRFTDTR KDEQERCITIKSTAISLFYE LSENDLNFIKQSKDGAGFLI NLIDSPGHVDFSSEVTAALR VTDGALVVVDCVSGVCVQTE TVLRQAIAERIKPVLMMNKM DRALLELQLEPEELYQTFQR IVENVNVIISTYGEGESGPM GNIMIDPVLGTVGFGSGLHG WAFTLKQFAEMYVAKFAAK |
| Post Translational Modifications | Phosphorylation by EF-2 kinase completely inactivates EF-2.it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment. Diphthamide is 2-3-carboxyamido-3-(trimethyl-ammonio)propylhistidine. (Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. ISGylated. Proteolytically processed at two sites following phosphorylation by CSK. SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage. |
| Function | Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. |
| Protein Name | Elongation Factor 2 Ef-2 |
| Database Links | Reactome: R-HSA-156902 Reactome: R-HSA-5336415 Reactome: R-HSA-5358493 Reactome: R-HSA-6798695 Reactome: R-HSA-8876725 |
| Cellular Localisation | Cytoplasm Nucleus Phosphorylation By Csk Promotes Cleavage And Sumoylation-Dependent Nuclear Translocation Of The C-Terminal Cleavage Product |
| Alternative Antibody Names | Anti-Elongation Factor 2 antibody Anti-Ef-2 antibody Anti-EEF2 antibody Anti-EF2 antibody |
Information sourced from Uniprot.org