• Immunohistochemistry analysis of paraffin-embedded human stomach using DTNBP1 antibody (STJ119008) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using DTNBP1 antibody (STJ119008) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-DTNBP1 antibody (1-351) (STJ119008)

SKU:
STJ119008

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DTNBP1 (1-351) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DTNBP1
Gene ID: 84062
Uniprot ID: DTBP1_HUMAN
Immunogen Region: 1-351
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human DTNBP1 (NP_115498.2).
Immunogen Sequence: MLETLRERLLSVQQDFTSGL KTLSDKSREAKVKSKPRTVP FLPKYSAGLELLSRYEDTWA ALHRRAKDCASAGELVDSEV VMLSAHWEKKKTSLVELQEQ LQQLPALIADLESMTANLTH LEASFEEVENNLLHLEDLCG QCELERCKHMQSQQLENYKK NKRKELETFKAELDAEHAQK VLEMEHTQQMKLKERQKFFE EAFQQDMEQYLSTGYLQIAE RREPIGSMSSMEVNVDMLE
Tissue Specificity Detected in brain, in neurons and in neuropil. Isoform 1 is expressed in the cerebral cortex, and hippocampal frontal (HF). Specific expression in the posterior half of the superior temporal gyrus (pSTG). Higher expression of isoform 2 and 3 in the HF than in the pSTG while isoform 1 shows no difference in expression in these areas. In the HF, detected in dentate gyrus (DG) and in pyramidal cells of hippocampus CA2 and CA3 (at protein level). Expressed in all principal neuronal populations of the HF, namely pyramidal neurons in the subiculum and CA1-3, granule cells in the dense cell layer of the DG (DGg), and polymorph cells in the hilus of the DG (DGh). Maximal levels in CA2, CA3, and DGh. Isoform 2 not expressed in the cerebral cortex.
Post Translational Modifications Ubiquitinated by TRIM32. Ubiquitination leads to DTNBP1 degradation. Isoforms 1 and 2 highly phosphorylated by PRKDC in vitro. Isoform 3 only weakly phosphorylated by PRKDC in vitro.
Function Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Associates with the BLOC-2 complex to facilitate the transport of TYRP1 independent of AP-3 function. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. Plays a role in the regulation of cell surface exposure of DRD2. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Appears to promote neuronal transmission and viability through regulating the expression of SNAP25 and SYN1, modulating PI3-kinase-Akt signaling and influencing glutamatergic release. Regulates the expression of SYN1 through binding to its promoter. Modulates prefrontal cortical activity via the dopamine/D2 pathway.
Protein Name Dysbindin
Biogenesis Of Lysosome-Related Organelles Complex 1 Subunit 8
Bloc-1 Subunit 8
Dysbindin-1
Dystrobrevin-Binding Protein 1
Hermansky-Pudlak Syndrome 7 Protein
Hps7 Protein
Database Links Reactome: R-HSA-432722
Cellular Localisation Isoform 1: Cytoplasm
Cytoplasmic Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Endosome Membrane
Melanosome Membrane
Postsynaptic Density
Endoplasmic Reticulum
Nucleus
Mainly Cytoplasmic But Shuttles Between The Cytoplasm And Nucleus
Exported Out Of The Nucleus Via Its Nes In A Xpo1-Dependent Manner
Nuclear Localization Is Required For Regulation Of The Expression Of Genes Such As Syn1
Detected In Neuron Cell Bodies
Axons And Dendrites
Mainly Located To The Postsynaptic Density
Detected At Tubulovesicular Elements In The Vicinity Of The Golgi Apparatus And Of Melanosomes
Occasionally Detected At The Membrane Of Pigmented Melanosomes In Cultured Melanoma Cells
The Bloc-1 Complex Associates With The Bloc-2 Complex In Early Endosome-Associated Tubules
Isoform 2: Cytoplasm
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Postsynaptic Cell Membrane
Shuttles Between The Cytoplasm And Nucleus
Mainly Expressed In The Dendritic Spine
Predominantly A Synaptic Vesicle Isoform But Also Highly Expressed In The Nucleus
Associated With The Ap-3 Complex At Presynaptic Terminals
Isoform 3: Cytoplasm
Exclusivley Cytoplasmic
Predominantly Found In The Postsynaptic Density (Psd)
Little Association With Synaptic Vesicles
Alternative Antibody Names Anti-Dysbindin antibody
Anti-Biogenesis Of Lysosome-Related Organelles Complex 1 Subunit 8 antibody
Anti-Bloc-1 Subunit 8 antibody
Anti-Dysbindin-1 antibody
Anti-Dystrobrevin-Binding Protein 1 antibody
Anti-Hermansky-Pudlak Syndrome 7 Protein antibody
Anti-Hps7 Protein antibody
Anti-DTNBP1 antibody
Anti-BLOC1S8 antibody
Anti-My031 antibody

Information sourced from Uniprot.org

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