| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DNASE1L3 (21-281) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DNASE1L3 |
| Gene ID: |
1776 |
| Uniprot ID: |
DNSL3_HUMAN |
| Immunogen Region: |
21-281 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 21-281 of human DNASE1L3 (NP_004935.1). |
| Immunogen Sequence: |
MRICSFNVRSFGESKQEDKN AMDVIVKVIKRCDIILVMEI KDSNNRICPILMEKLNRNSR RGITYNYVISSRLGRNTYKE QYAFLYKEKLVSVKRSYHYH DYQDGDADVFSREPFVVWFQ SPHTAVKDFVIIPLHTTPET SVKEIDELVEVYTDVKHRWK AENFIFMGDFNAGCSYVPKK AWKNIRLRTDPRFVWLIGDQ EDTTVKKSTNCAYDRIVLRG QEIVSSVVPKSNSVFDFQK |
| Tissue Specificity | Liver and spleen. |
| Post Translational Modifications | Poly-ADP-ribosylated by PARP1. ADP-ribosylation negatively regulates enzymatic activity during apoptosis. |
| Function | Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells. Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity. Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation. |
| Protein Name | Deoxyribonuclease GammaDnase GammaDnase I Homolog Protein Dhp2Deoxyribonuclease I-Like 3Dnase I-Like 3Liver And Spleen DnaseLs-DnaseLsd |
| Cellular Localisation | NucleusEndoplasmic ReticulumSecretedTranslocates From The Endoplasmic Reticulum To The Nucleus During ApoptosisContradictory Reports Exist About The Subcellular Localization Under Normal Physiological ConditionsUnder Conditions Of Cell DeathMay Diffuse And/Or Be Actively Transported To The Nucleus |
| Alternative Antibody Names | Anti-Deoxyribonuclease Gamma antibodyAnti-Dnase Gamma antibodyAnti-Dnase I Homolog Protein Dhp2 antibodyAnti-Deoxyribonuclease I-Like 3 antibodyAnti-Dnase I-Like 3 antibodyAnti-Liver And Spleen Dnase antibodyAnti-Ls-Dnase antibodyAnti-Lsd antibodyAnti-DNASE1L3 antibodyAnti-DHP2 antibodyAnti-DNAS1L3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
