| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DNAJB9 (24-223) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DNAJB9 |
| Gene ID: |
4189 |
| Uniprot ID: |
DNJB9_HUMAN |
| Immunogen Region: |
24-223 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 24-223 of human DNAJB9 (NP_036460.1). |
| Immunogen Sequence: |
SKSYYDILGVPKSASERQIK KAFHKLAMKYHPDKNKSPDA EAKFREIAEAYETLSDANRR KEYDTLGHSAFTSGKGQRGS GSSFEQSFNFNFDDLFKDFG FFGQNQNTGSKKRFENHFQT RQDGGSSRQRHHFQEFSFGG GLFDDMFEDMEKMFSFSGFD STNQHTVQTENRFHGSSKHC RTVTQRRGNMVTTYTDCSGQ |
| Tissue Specificity | Widely expressed. Expressed at highest level in the liver, placenta and kidney. |
| Function | Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins. In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP: HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1. Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production. |
| Protein Name | Dnaj Homolog Subfamily B Member 9Endoplasmic Reticulum Dna J Domain-Containing Protein 4Er-Resident Protein Erdj4Erdj4Microvascular Endothelial Differentiation Gene 1 ProteinMdg-1 |
| Database Links | Reactome: R-HSA-381038 |
| Cellular Localisation | Endoplasmic Reticulum Lumen |
| Alternative Antibody Names | Anti-Dnaj Homolog Subfamily B Member 9 antibodyAnti-Endoplasmic Reticulum Dna J Domain-Containing Protein 4 antibodyAnti-Er-Resident Protein Erdj4 antibodyAnti-Erdj4 antibodyAnti-Microvascular Endothelial Differentiation Gene 1 Protein antibodyAnti-Mdg-1 antibodyAnti-DNAJB9 antibodyAnti-MDG1 antibodyAnti-UNQ743 antibodyAnti-PRO1471 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89668/358164/STJ11100730_1__42931.1713122467.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")