• Immunofluorescence analysis of U2OS cells using DNAJB6 Rabbit polyclonal antibody (STJ29132). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of DNAJB6 in paraffin-embedded mouse ileum using DNAJB6 Rabbit polyclonal antibody (STJ29132) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DNAJB6 in paraffin-embedded human kidney cancer using DNAJB6 Rabbit polyclonal antibody (STJ29132) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using DNAJB6 Rabbit polyclonal antibody (STJ29132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DNAJB6 antibody (177-326) (STJ29132)

SKU:
STJ29132

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNAJB6 (177-326) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNAJB6
Gene ID: 10049
Uniprot ID: DNJB6_HUMAN
Immunogen Region: 177-326
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).
Immunogen Sequence: STSFGGSGMGNFKSISTSTK MVNGRKITTKRIVENGQERV EVEEDGQLKSLTINGVADDD ALAEERMRRGQNALPAQPAG LRPPKPPRPASLLRHAPHCL SEEEGEQDRPRAPGPWDPLA SAAGLKEGGKRKKQKQREES KKKKSTKGNH
Tissue Specificity Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.
Function Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Also reduces cellular toxicity and caspase-3 activity. Isoform B: Isoform B but not isoform A inhibits huntingtin aggregation.
Protein Name Dnaj Homolog Subfamily B Member 6
Hhdj1
Heat Shock Protein J2
Hsj-2
Mrj
Msj-1
Database Links Reactome: R-HSA-3371453
Cellular Localisation Cytoplasm
Perinuclear Region
Nucleus
Myofibril
Sarcomere
Z Line
Alternative Antibody Names Anti-Dnaj Homolog Subfamily B Member 6 antibody
Anti-Hhdj1 antibody
Anti-Heat Shock Protein J2 antibody
Anti-Hsj-2 antibody
Anti-Mrj antibody
Anti-Msj-1 antibody
Anti-DNAJB6 antibody
Anti-HSJ2 antibody
Anti-MRJ antibody
Anti-MSJ1 antibody

Information sourced from Uniprot.org

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