| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Dna Replication Atp-Dependent Helicase/Nuclease Dna2 (811-1060) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DNA2 |
| Gene ID: |
1763 |
| Uniprot ID: |
DNA2_HUMAN |
| Immunogen Region: |
811-1060 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 811-1060 of human DNA2 (NP_001073918.2). |
| Immunogen Sequence: |
KRLEQNKSAVVQLTVQYRMN SKIMSLSNKLTYEGKLECGS DKVANAVINLRHFKDVKLEL EFYADYSDNPWLMGVFEPNN PVCFLNTDKVPAPEQVEKGG VSNVTEAKLIVFLTSIFVKA GCSPSDIGIIAPYRQQLKII NDLLARSIGMVEVNTVDKYQ GRDKSIVLVSFVRSNKDGTV GELLKDWRRLNVAITRAKHK LILLGCVPSLNCYPPLEKLL NHLNSEKLIIDLPSREHES |
| Post Translational Modifications | Acetylated by EP300, leading to stimulate the 5'-3' endonuclease, the 5'-3' helicase and DNA-dependent ATPase activities, possibly by increasing DNA substrate affinity. |
| Function | Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function. |
| Protein Name | Dna Replication Atp-Dependent Helicase/Nuclease Dna2Hdna2Dna Replication Atp-Dependent Helicase-Like Homolog Includes - Dna Replication Nuclease Dna2 - Dna Replication Atp-Dependent Helicase Dna2 |
| Database Links | Reactome: R-HSA-174437Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69166Reactome: R-HSA-69473Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusMitochondrionWas Initially Reported To Be Exclusively MitochondrialHoweverIt Was Later Shown To Localize Both In Mitochondrion And Nucleus |
| Alternative Antibody Names | Anti-Dna Replication Atp-Dependent Helicase/Nuclease Dna2 antibodyAnti-Hdna2 antibodyAnti-Dna Replication Atp-Dependent Helicase-Like Homolog Includes - Dna Replication Nuclease Dna2 - Dna Replication Atp-Dependent Helicase Dna2 antibodyAnti-DNA2 antibodyAnti-DNA2L antibodyAnti-KIAA0083 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
