Western blot analysis of extracts of Jurkat cells, using DNA Ligase I antibody (STJ11103656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DNA Ligase I antibody (1-91) [S6MR] (STJ11103656)

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STJ11103656
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIG1
Gene ID: 3978
Uniprot ID: DNLI1_HUMAN
Immunogen Region: 1-91
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-91 of human DNA Ligase I (P18858).
Immunogen Sequence: MQRSIMSFFHPKKEGKAKKP EKEASNSSRETEPPPKAALK EWNGVVSESDSPVKRPGRKA ARVLGSEGEEEDEALSPAKG QKPALDCSQVS
Function DNA ligase that seals nicks in double-stranded during DNA repair. Also involved in DNA replication and DNA recombination.
Protein Name Dna Ligase 1
Dna Ligase I
Polydeoxyribonucleotide Synthase Atp 1
Database Links Reactome: R-HSA-110362
Reactome: R-HSA-162594
Reactome: R-HSA-174414
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5696397
Reactome: R-HSA-6782210
Reactome: R-HSA-69183
Reactome: R-HSA-9825895
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Ligase 1 antibody
Anti-Dna Ligase I antibody
Anti-Polydeoxyribonucleotide Synthase Atp 1 antibody
Anti-LIG1 antibody

Information sourced from Uniprot.org

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