• Immunofluorescence analysis of paraffin-embedded human skin cancer using DKK2 Rabbit polyclonal antibody (STJ117074) at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using DKK2 Rabbit polyclonal antibody (STJ117074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DKK2 antibody (C-Term) (STJ117074)

SKU:
STJ117074

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DKK2 (C-Term) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DKK2
Gene ID: 27123
Uniprot ID: DKK2_HUMAN
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DKK2 (NP_055236.1).
Immunogen Sequence: CMVCRRKKKRCHRDGMCCPS TRCNNGICIPVTESILTPHI PALDGTRHRDRNHGHYSNHD LGWQNLGRPHTKMSHIKGHE GDPCLRSSDCIEGFCCARHF WTKICKPVLHQGEVCTKQRK KGSHGLEIFQRCDCAKGLSC KVWKDATYSSKARLHVCQKI
Tissue Specificity Expressed in heart, brain, skeletal muscle and lung.
Post Translational Modifications May be proteolytically processed by a furin-like protease.
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Protein Name Dickkopf-Related Protein 2
Dickkopf-2
Dkk-2
Hdkk-2
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Reactome: R-HSA-5339717
Cellular Localisation Secreted
Alternative Antibody Names Anti-Dickkopf-Related Protein 2 antibody
Anti-Dickkopf-2 antibody
Anti-Dkk-2 antibody
Anti-Hdkk-2 antibody
Anti-DKK2 antibody
Anti-UNQ682 antibody
Anti-PRO1316 antibody

Information sourced from Uniprot.org

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