• Immunohistochemistry analysis of DKC1 in paraffin-embedded human spleen tissue using DKC1 Rabbit monoclonal antibody (STJ11102073) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DKC1 in paraffin-embedded rat spleen tissue using DKC1 Rabbit monoclonal antibody (STJ11102073) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DKC1 in paraffin-embedded mouse testis tissue using DKC1 Rabbit monoclonal antibody (STJ11102073) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DKC1 in paraffin-embedded rat colon tissue using DKC1 Rabbit monoclonal antibody (STJ11102073) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DKC1 in paraffin-embedded mouse brain tissue using DKC1 Rabbit monoclonal antibody (STJ11102073) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DKC1 in paraffin-embedded human cervix cancer tissue using DKC1 Rabbit monoclonal antibody (STJ11102073) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DKC1 in paraffin-embedded mouse colon tissue using DKC1 Rabbit monoclonal antibody (STJ11102073) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using DKC1 Rabbit monoclonal antibody (STJ11102073) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from PC-3 cells, using DKC1 Rabbit monoclonal antibody (STJ11102073) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DKC1 antibody (300-400) [S3MR] (STJ11102073)

SKU:
STJ11102073

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DKC1 (300-400) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DKC1
Gene ID: 1736
Uniprot ID: DKC1_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human DKC1 (O60832).
Immunogen Sequence: VMKDSAVNAICYGAKIMLPG VLRYEDGIEVNQEIVVITTK GEAICMAIALMTTAVISTCD HGIVAKIKRVIMERDTYPRK WGLGPKASQKKLMIKQGLLD K
Tissue Specificity Ubiquitously expressed.
Function Isoform 1: Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Required for ribosome biogenesis and telomere maintenance. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression.
Protein Name H/Aca Ribonucleoprotein Complex Subunit Dkc1
Cbf5 Homolog
Dyskerin
Nopp140-Associated Protein Of 57 Kda
Nucleolar Protein Nap57
Nucleolar Protein Family A Member 4
Snornp Protein Dkc1
Database Links Reactome: R-HSA-171319
Reactome: R-HSA-6790901
Cellular Localisation Isoform 1: Nucleus
Nucleolus
Nucleus
Cajal Body
Also Localized To Cajal Bodies (Coiled Bodies)
Isoform 3: Cytoplasm
Alternative Antibody Names Anti-H/Aca Ribonucleoprotein Complex Subunit Dkc1 antibody
Anti-Cbf5 Homolog antibody
Anti-Dyskerin antibody
Anti-Nopp140-Associated Protein Of 57 Kda antibody
Anti-Nucleolar Protein Nap57 antibody
Anti-Nucleolar Protein Family A Member 4 antibody
Anti-Snornp Protein Dkc1 antibody
Anti-DKC1 antibody
Anti-NOLA4 antibody

Information sourced from Uniprot.org

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