• Western blot analysis of extracts of various cell lines, using DICER1 antibody (STJ27817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry of paraffin-embedded mouse brain using DICER1 rabbit polyclonal antibody (STJ27817) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded Rat brain using DICER1 rabbit polyclonal antibody (STJ27817) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded Human placenta using DICER1 rabbit polyclonal antibody (STJ27817) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of L929 cells using DICER1 Polyclonal Antibody (STJ27817) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of SH-SY5Y cells using 3ug DICER1 antibody (STJ27817). Western blot was performed from the immunoprecipitate using DICER1 antibody (STJ27817) at a dilition of 1:500.

Anti-DICER1 antibody (1200-1460) (STJ27817)

SKU:
STJ27817

€211.88 - €561.88
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DICER1 (1200-1460) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:100-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DICER1
Gene ID: 23405
Uniprot ID: DICER_HUMAN
Immunogen Region: 1200-1460
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1460 of human DICER1 (NP_085124.2).
Immunogen Sequence: NQLNYYKQEIPVQPTTSYSI QNLYSYENQPQPSDECTLLS NKYLDGNANKSTSDGSPVMA VMPGTTDTIQVLKGRMDSEQ SPSIGYSSRTLGPNPGLILQ ALTLSNASDGFNLERLEMLG DSFLKHAITTYLFCTYPDAH EGRLSYMRSKKVSNCNLYRL GKKKGLPSRMVVSIFDPPVN WLPPGYVVNQDKSNTDKWEK DEMTKDCMLANGKLDEDYEE EDEEEESLMWRAPKEEADY
Function Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
Protein Name Endoribonuclease Dicer
Helicase With Rnase Motif
Helicase Moi
Database Links Reactome: R-HSA-203927
Reactome: R-HSA-426486
Reactome: R-HSA-9708296
Cellular Localisation Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Endoribonuclease Dicer antibody
Anti-Helicase With Rnase Motif antibody
Anti-Helicase Moi antibody
Anti-DICER1 antibody
Anti-DICER antibody
Anti-HERNA antibody
Anti-KIAA0928 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance