| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DEPDC6 (1-220) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DEPTOR |
| Gene ID: |
64798 |
| Uniprot ID: |
DPTOR_HUMAN |
| Immunogen Region: |
1-220 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human DEPDC6 (NP_073620.2). |
| Immunogen Sequence: |
MEEGGSTGSAGSDSSTSGSG GAQQRELERMAEVLVTGEQL RLRLHEEKVIKDRRHHLKTY PNCFVAKELIDWLIEHKEAS DRETAIKLMQKLADRGIIHH VCDEHKEFKDVKLFYRFRKD DGTFPLDNEVKAFMRGQRLY EKLMSPENTLLQPREEEGVK YERTFMASEFLDWLVQEGEA TTRKEAEQLCHRLMEHGIIQ HVSNKHPFVDSNLLYQFRMN |
| Post Translational Modifications | Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2. Phosphorylated at Ser-286, Ser-287 and Ser-291 in response to mitogenic stimulation by MTOR: DEPTOR is either directly phosphorylated by MTOR or indirectly via proteins kinases that are activated by MTOR, such as CK1/CSNK1A1. Phosphorylation at Ser-286, Ser-287 and Ser-291 promotes ubiquitination by the SCF(BTRC) complex, followed by degradation. Phosphorylation at Ser-235 by MAPK3/ERK1 promotes deubiquitination by USP7, enhancing its stability. Phosphorylation at Tyr-289 by SYK impairs its interaction with MTOR, promoting mTORC1 and mTORC2 signaling. Ubiquitinated.leading to proteasomal degradation. Ubiquitination by the SCF(BTRC) and SCF(FBXW11) complexes following phosphorylation at Ser-286, Ser-287 and Ser-291 by MTOR, leads to its degradation by the proteasome. Deubiquitinated by OTUB1 in response to amino acid via a non-canonical mechanism, leading to DEPTOR stability. Deubiquitinated by USP7 following phosphorylation at Ser-235, promoting its stability. |
| Function | Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes. DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy. In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity. |
| Protein Name | Dep Domain-Containing Mtor-Interacting ProteinHdeptorDep Domain-Containing Protein 6 |
| Cellular Localisation | Lysosome MembraneLocalizes To The Lysosomal Membrane When Associated With The Mtorc1 And Mtorc2 Complexes |
| Alternative Antibody Names | Anti-Dep Domain-Containing Mtor-Interacting Protein antibodyAnti-Hdeptor antibodyAnti-Dep Domain-Containing Protein 6 antibodyAnti-DEPTOR antibodyAnti-DEPDC6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
