• Western blot analysis of lysates from mouse testis, using DDI1 Rabbit polyclonal antibody (STJ116678) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DDI1 antibody (97-396) (STJ116678)

SKU:
STJ116678

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDI1 (97-396) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDI1
Gene ID: 414301
Uniprot ID: DDI1_HUMAN
Immunogen Region: 97-396
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 97-396 of human DDI1 (NP_001001711.1).
Immunogen Sequence: SGIAVPGTSSSRPQHPGQQQ QRTPAAQRSQGLASGEKVAG LQGLGSPALIRSMLLSNPHD LSLLKERNPPLAEALLSGSL ETFSQVLMEQQREKALREQE RLRLYTADPLDREAQAKIEE EIRQQNIEENMNIAIEEAPE SFGQVTMLYINCKVNGHPLK AFVDSGAQMTIMSQACAERC NIMRLVDRRWAGVAKGVGTQ RIIGRVHLAQIQIEGDFLQC SFSILEDQPMDMLLGLDML
Function Probable aspartic protease (Probable). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI2, for cellular survival following replication stress. Together or redudantly with DDI2, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity.
Protein Name Protein Ddi1 Homolog 1
Alternative Antibody Names Anti-Protein Ddi1 Homolog 1 antibody
Anti-DDI1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance