| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC/IF/FC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-DAG1 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IHC-5µg/mlELISA-antibody detection limit dilution 1:128000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
DAG1 |
| Gene ID: |
1605 |
| Uniprot ID: |
DAG1_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_004384.4 |
| Specificity: |
This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan. |
| Immunogen Sequence: |
HVGKHEYFMHATDK |
| Post Translational Modifications | Alpha-dystroglycan: O-glycosylated. POMGNT1 catalyzes the initial addition of N-acetylglucosamine, giving rise to the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety and thus providing the necessary basis for the addition of further carbohydrate moieties. Heavily O-glycosylated comprising of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. Mucin-type O-glycosylation is important for ligand binding activity. O-mannosylation is found in high abundance in both brain and muscle where the most abundant glycan is Sia-alpha-2-3-Gal-beta-1-4-Glc-NAc-beta-1-2-Man. In muscle, glycosylation on Thr-317, Thr-319 and Thr-379 by a phosphorylated O-mannosyl glycan with the structure 2-(N-acetylamido)-2-deoxygalactosyl-beta-1,3-2-(N-acetylamido)-2-deoxyglucosyl-beta-1,4-6-phosphomannose is mediated by like-acetylglucosaminyltransferase (LARGE1) protein and is required for laminin binding. The O-glycosyl hexose on Thr-367, Thr-369, Thr-372, Thr-381 and Thr-388 is probably mannose. O-glycosylated in the N-terminal region with a core 1 or possibly core 8 glycan. The brain form displays a unique glycosylation pattern which is absent in other tissues.this form shows enhanced binding to laminin LAMA5 compared to the skeletal muscle form. Alpha-dystroglycan: (Microbial infection) O-mannosylation is required for binding lymphocytic choriomeningitis virus, Old World Lassa fever virus, and clade C New World arenaviruses. Beta-dystroglycan: N-glycosylated. Autolytic cleavage produces the alpha and beta subunits. In cutaneous cells, as well as in certain pathological conditions, shedding of beta-dystroglycan can occur releasing a peptide of about 30 kDa. SRC-mediated phosphorylation of the PPXY motif of the beta subunit recruits SH2 domain-containing proteins, but inhibits binding to WWW domain-containing proteins, DMD and UTRN. This phosphorylation also inhibits nuclear entry. |
| Function | The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Alpha-dystroglycan: Extracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Also acts as a receptor for laminin LAMA5. Beta-dystroglycan: Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity. Alpha-dystroglycan: (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus glycoprotein and class C new-world arenaviruses. Acts as a Schwann cell receptor for Mycobacterium leprae, the causative organism of leprosy, but only in the presence of the G-domain of LAMA2. |
| Protein Name | Dystroglycan 1DystroglycanDystrophin-Associated Glycoprotein 1 Cleaved Into - Alpha-DystroglycanAlpha-Dg - Beta-DystroglycanBeta-Dg |
| Database Links | Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-5083628Reactome: R-HSA-5083629Reactome: R-HSA-5083633Reactome: R-HSA-5173105Reactome: R-HSA-9010553Reactome: R-HSA-9619665 |
| Cellular Localisation | Alpha-Dystroglycan: SecretedExtracellular SpaceBeta-Dystroglycan: Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmCytoskeletonNucleusNucleoplasmCell MembraneSarcolemmaPostsynaptic Cell MembraneThe Monomeric Form Translocates To The Nucleus Via The Action Of Importins And Depends On RanNuclear Transport Is Inhibited By Tyr-892 PhosphorylationIn Skeletal MuscleThis Phosphorylated Form Locates To A Vesicular Internal Membrane CompartmentIn Muscle CellsSarcolemma Localization Requires The Presence Of Ank2While Localization To Costameres Requires The Presence Of Ank3Localizes To Neuromuscular Junctions (Nmjs) In The Presence Of Ank2In Peripheral NervesLocalizes To The Schwann Cell MembraneColocalizes With Erm Proteins In Schwann-Cell Microvilli |
| Alternative Antibody Names | Anti-Dystroglycan 1 antibodyAnti-Dystroglycan antibodyAnti-Dystrophin-Associated Glycoprotein 1 Cleaved Into - Alpha-Dystroglycan antibodyAnti-Alpha-Dg - Beta-Dystroglycan antibodyAnti-Beta-Dg antibodyAnti-DAG1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
