• Immunofluorescence analysis of A431 cells using CYR61 Rabbit polyclonal antibody (STJ11104356) at dilution of 200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat lung, using (STJ11104356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using (STJ11104356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CYR61 antibody (120-220) (STJ11104356)

SKU:
STJ11104356

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYR61 (120-220) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCN1
Gene ID: 3491
Uniprot ID: CCN1_HUMAN
Immunogen Region: 120-220
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 120-220 of human CYR61 (NP_001545.2).
Immunogen Sequence: QCTCIDGAVGCIPLCPQELS LPNLGCPNPRLVKVTGQCCE EWVCDEDSIKDPMEDQDGLL GKELGFDASEVELTRNNELI AVGKGSSLKRLPVFGMEPRI L
Function Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Protein Name Ccn Family Member 1
Cellular Communication Network Factor 1
Cysteine-Rich Angiogenic Inducer 61
Insulin-Like Growth Factor-Binding Protein 10
Ibp-10
Igf-Binding Protein 10
Igfbp-10
Protein Cyr61
Protein Gig1
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Ccn Family Member 1 antibody
Anti-Cellular Communication Network Factor 1 antibody
Anti-Cysteine-Rich Angiogenic Inducer 61 antibody
Anti-Insulin-Like Growth Factor-Binding Protein 10 antibody
Anti-Ibp-10 antibody
Anti-Igf-Binding Protein 10 antibody
Anti-Igfbp-10 antibody
Anti-Protein Cyr61 antibody
Anti-Protein Gig1 antibody
Anti-CCN1 antibody
Anti-CYR61 antibody
Anti-GIG1 antibody
Anti-IGFBP10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance