| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CXCL12 |
| Gene ID: | 6387 |
| Uniprot ID: | SDF1_HUMAN |
| Immunogen Region: | 40-93 aa |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 40-93 of human CXCL12 (NP_000600.1). |
| Immunogen Sequence: | ARANVKHLKILNTPNCALQI VARLKNNNRQVCIDPKLKWI QEYLEKALNKRFKM |
| Tissue Specificity | Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. |
| Post Translational Modifications | Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. |
| Function | Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development.required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells. |
| Protein Name | Stromal Cell-Derived Factor 1 Sdf-1 Hsdf-1 C-X-C Motif Chemokine 12 Intercrine Reduced In Hepatomas Irh Hirh Pre-B Cell Growth-Stimulating Factor Pbsf Cleaved Into - Sdf-1-Beta(3-72 - Sdf-1-Alpha(3-67 |
| Database Links | Reactome: R-HSA-1251985 Reactome: R-HSA-376176 Reactome: R-HSA-380108 Reactome: R-HSA-418594 Reactome: R-HSA-9018519 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Stromal Cell-Derived Factor 1 antibody Anti-Sdf-1 antibody Anti-Hsdf-1 antibody Anti-C-X-C Motif Chemokine 12 antibody Anti-Intercrine Reduced In Hepatomas antibody Anti-Irh antibody Anti-Hirh antibody Anti-Pre-B Cell Growth-Stimulating Factor antibody Anti-Pbsf Cleaved Into - Sdf-1-Beta(3-72 - Sdf-1-Alpha(3-67 antibody Anti-CXCL12 antibody Anti-SDF1 antibody Anti-SDF1A antibody Anti-SDF1B antibody |
Information sourced from Uniprot.org