• Immunohistochemistry analysis of paraffin-embedded human tonsil using Cathepsin D Rabbit polyclonal antibody (STJ115256) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using Cathepsin D Rabbit polyclonal antibody (STJ115256) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of Rat lung, using CathepsinD antibody (STJ115256) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of SK-BR-3, using CathepsinD antibody (STJ115256) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CTSD antibody (21-249) (STJ115256)

SKU:
STJ115256

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTSD (21-249) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTSD
Gene ID: 1509
Uniprot ID: CATD_HUMAN
Immunogen Region: 21-249
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-249 of human Cathepsin D (NP_001900.1).
Immunogen Sequence: LVRIPLHKFTSIRRTMSEVG GSVEDLIAKGPVSKYSQAVP AVTEGPIPEVLKNYMDAQYY GEIGIGTPPQCFTVVFDTGS SNLWVPSIHCKLLDIACWIH HKYNSDKSSTYVKNGTSFDI HYGSGSLSGYLSQDTVSVPC QSASSASALGGVKVERQVFG EATKQPGITFIAAKFDGILG MAYPRISVNNVLPVFDNLMQ QKLVDQNIFSFYLSRDPDAQ PGGELMLGG
Tissue Specificity Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).
Post Translational Modifications N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.
Function Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Protein Name Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-2022377
Reactome: R-HSA-2132295
Reactome: R-HSA-6798695
Reactome: R-HSA-77387
Reactome: R-HSA-9018519
Cellular Localisation Lysosome
Melanosome
Secreted
Extracellular Space
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
In Aortic Samples
Detected As An Extracellular Protein Loosely Bound To The Matrix
Alternative Antibody Names Anti-Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain antibody
Anti-CTSD antibody
Anti-CPSD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance