• Western blot analysis of extracts of BT-474 cells, using CST4 antibody (STJ110413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of extracts of BT-474 cells, using CST4 antibody (STJ110413) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CST4 antibody (21-141) (STJ110413)

SKU:
STJ110413

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CST4
Gene ID: 1472
Uniprot ID: CYTS_HUMAN
Immunogen Region: 21-141
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 21-141 of human CST4 (NP_001890.1).
Immunogen Sequence: SSSKEENRIIPGGIYDADLN DEWVQRALHFAISEYNKATE DEYYRRPLQVLRAREQTFGG VNYFFDVEVGRTICTKSQPN LDTCAFHEQPELQKKQLCSF EIYEVPWEDRMSLVNSRCQE A
Tissue Specificity Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid.
Post Translational Modifications Phosphorylated at both its N- and C-terminal regions.
Function This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.
Protein Name Cystatin-S
Cystatin-4
Cystatin-Sa-Iii
Salivary Acidic Protein 1
Cellular Localisation Secreted
Alternative Antibody Names Anti-Cystatin-S antibody
Anti-Cystatin-4 antibody
Anti-Cystatin-Sa-Iii antibody
Anti-Salivary Acidic Protein 1 antibody
Anti-CST4 antibody

Information sourced from Uniprot.org

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