Western blot analysis of extracts of various cell lines, using CRHR2 antibody (STJ114302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CRHR2 antibody (25-140) (STJ114302)

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STJ114302
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRHR2
Gene ID: 1395
Uniprot ID: CRFR2_HUMAN
Immunogen Region: 25-140
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 25-140 of human CRHR2 (NP_001189404.1).
Immunogen Sequence: PPPLQYAAGQSQMPKDQPLW ALLEQYCHTIMTLTNLSGPY SYCNTTLDQIGTCWPRSAAG ALVERPCPEYFNGVKYNTTR NAYRECLENGTWASKINYSQ CEPILDDKQRKYDLHY
Post Translational Modifications A N-glycosylation site within the signal peptide impedes its proper cleavage and function.
Function G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.
Protein Name Corticotropin-Releasing Factor Receptor 2
Crf-R-2
Crf-R2
Crfr-2
Corticotropin-Releasing Hormone Receptor 2
Crh-R-2
Crh-R2
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-418555
Reactome: R-HSA-422085
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Corticotropin-Releasing Factor Receptor 2 antibody
Anti-Crf-R-2 antibody
Anti-Crf-R2 antibody
Anti-Crfr-2 antibody
Anti-Corticotropin-Releasing Hormone Receptor 2 antibody
Anti-Crh-R-2 antibody
Anti-Crh-R2 antibody
Anti-CRHR2 antibody
Anti-CRF2R antibody
Anti-CRH2R antibody

Information sourced from Uniprot.org

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