• Immunofluorescence analysis of paraffin-embedded rat trachea using COL2A1 Rabbit polyclonal antibody (STJ11103919) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse skeletal muscle, using COL2A1 Rabbit polyclonal antibody (STJ11103919) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-COL2A1 antibody (1200-1300) (STJ11103919)

SKU:
STJ11103919

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Collagen Alpha-1 (Ii Chain (1200-1300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL2A1
Gene ID: 1280
Uniprot ID: CO2A1_HUMAN
Immunogen Region: 1200-1300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of human COL2A1 (NP_001835.3).
Immunogen Sequence: GPPGNPGPPGPPGPPGPGID MSAFAGLGPREKGPDPLQYM RADQAAGGLRQHDAEVDATL KSLNNQIESIRSPEGSRKNP ARTCRDLKLCHPEWKSGDYW I
Tissue Specificity Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.
Post Translational Modifications The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2. Contains mostly 4-hydroxyproline. Prolines at the third position of the tripeptide repeating unit (G-X-P) are 4-hydroxylated in some or all of the chains. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.
Function Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Protein Name Collagen Alpha-1(Ii Chain
Alpha-1 Type Ii Collagen Cleaved Into - Collagen Alpha-1(Ii Chain - Chondrocalcin
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-198933
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8874081
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(Ii Chain antibody
Anti-Alpha-1 Type Ii Collagen Cleaved Into - Collagen Alpha-1(Ii Chain - Chondrocalcin antibody
Anti-COL2A1 antibody

Information sourced from Uniprot.org

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