• Western blot analysis of extracts of various cell lines, using CNTN5 antibody (STJ114529) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CNTN5 antibody (19-220) (STJ114529)

SKU:
STJ114529

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CNTN5 (19-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNTN5
Gene ID: 53942
Uniprot ID: CNTN5_HUMAN
Immunogen Region: 19-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-220 of human CNTN5 (NP_001230200.1).
Immunogen Sequence: EYSKSLPGLSTSYAALLRIK KSSSSSLFGSKTRPRYSSPS LGTLSASSPSWLGAAQNYYS PINLYHSSDAFKQDESVDYG PVFVQEPDDIIFPTDSDEKK VALNCEVRGNPVPSYRWLRN GTEIDLESDYRYSLIDGTFI ISNPSEAKDSGHYQCLATNT VGSILSREATLQFAYLGNFS GRTRSAVSVREGQGVVLMCS PP
Tissue Specificity Expressed in brain and kidney and at very low level in placenta. Not expressed in other tissues. In brain, it is highly expressed in the occipital lobe, amygdala, cerebral cortex, frontal lobe, thalamus and temporal lobe. Expressed at moderate level in the cerebellum, substantia nigra, putamen, medulla and hippocampus. Weakly expressed in the spinal cord and caudate nucleus. Weakly or not expressed in the corpus callosum.
Function Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons. Probably involved in neuronal activity in the auditory system.
Protein Name Contactin-5
Neural Recognition Molecule Nb-2
Hnb-2
Database Links Reactome: R-HSA-163125
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Contactin-5 antibody
Anti-Neural Recognition Molecule Nb-2 antibody
Anti-Hnb-2 antibody
Anti-CNTN5 antibody

Information sourced from Uniprot.org

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