| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Cleaved-Glutathione hydrolase 1 proenzyme-Gly380, Heavy Chain (380-381) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GGT1 |
| Gene ID: | 2678 |
| Uniprot ID: | GGT1_HUMAN |
| Immunogen Region: | 380-381 |
| Specificity: | This antibody detects endogenous levels of Human GGT1 (heavy chain, Cleaved-Gly380, protein was cleaved amino acid sequence between 380-381 ) |
| Immunogen: | Synthesized peptide derived from human GGT1 (heavy chain, Cleaved-Gly380) |
| Tissue Specificity | Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein. Isoform 3: Lung-specific. |
| Post Translational Modifications | N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity. Cleaved by autocatalysis into a large and a small subunit and the autocatalytic cleavage is essential to the functional activation of the enzyme. |
| Function | Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4. Isoform 3: Seems to be inactive. |
| Protein Name | Glutathione Hydrolase 1 Proenzyme Gamma-Glutamyltransferase 1 Gamma-Glutamyltranspeptidase 1 Ggt 1 Leukotriene-C4 Hydrolase Cd Antigen Cd224 Cleaved Into - Glutathione Hydrolase 1 Heavy Chain - Glutathione Hydrolase 1 Light Chain |
| Database Links | Reactome: R-HSA-174403 Reactome: R-HSA-2142691 Reactome: R-HSA-5423646 Reactome: R-HSA-5579022 Reactome: R-HSA-9035968 Reactome: R-HSA-9664535 Reactome: R-HSA-9753281 |
| Cellular Localisation | Cell Membrane Single-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Glutathione Hydrolase 1 Proenzyme antibody Anti-Gamma-Glutamyltransferase 1 antibody Anti-Gamma-Glutamyltranspeptidase 1 antibody Anti-Ggt 1 antibody Anti-Leukotriene-C4 Hydrolase antibody Anti-Cd Antigen Cd224 Cleaved Into - Glutathione Hydrolase 1 Heavy Chain - Glutathione Hydrolase 1 Light Chain antibody Anti-GGT1 antibody Anti-GGT antibody |
Information sourced from Uniprot.org