• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CLDN4 antibody (STJ114778) at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using CLDN4 Rabbit polyclonal antibody (STJ114778) at 1:4000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : HL-60Exposure time: 30s.

Anti-CLDN4 antibody (109-209) (STJ114778)

SKU:
STJ114778

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLDN4 (109-209) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:4000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLDN4
Gene ID: 1364
Uniprot ID: CLD4_HUMAN
Immunogen Region: 109-209
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 109-209 of human CLDN4 (NP_001296.1).
Immunogen Sequence: EDESAKAKTMIVAGVVFLLA GLMVIVPVSWTAHNIIQDFY NPLVASGQKREMGASLYVGW AASGLLLLGGGLLCCNCPPR TDKPYSAKYSAARSAAASNY V
Post Translational Modifications Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1.
Function Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Protein Name Claudin-4
Clostridium Perfringens Enterotoxin Receptor
Cpe-R
Cpe-Receptor
Williams-Beuren Syndrome Chromosomal Region 8 Protein
Database Links Reactome: R-HSA-420029
Cellular Localisation Cell Junction
Tight Junction
Cell Membrane
Multi-Pass Membrane Protein
Cldn4 Is Required For Tight Junction Localization In The Kidney
Alternative Antibody Names Anti-Claudin-4 antibody
Anti-Clostridium Perfringens Enterotoxin Receptor antibody
Anti-Cpe-R antibody
Anti-Cpe-Receptor antibody
Anti-Williams-Beuren Syndrome Chromosomal Region 8 Protein antibody
Anti-CLDN4 antibody
Anti-CPER antibody
Anti-CPETR1 antibody
Anti-WBSCR8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance