• Immunofluorescence analysis of NIH/3T3 cells using Citrate synthetase Rabbit monoclonal antibody (STJ11103569) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Citrate synthetase in paraffin-embedded mouse kidney tissue using Citrate synthetase Rabbit monoclonal antibody (STJ11103569) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Citrate synthetase in paraffin-embedded human colon carcinoma tissue using Citrate synthetase Rabbit monoclonal antibody (STJ11103569) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Citrate synthetase in paraffin-embedded rat colon tissue using Citrate synthetase Rabbit monoclonal antibody (STJ11103569) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Citrate synthetase in paraffin-embedded rat liver tissue using Citrate synthetase Rabbit monoclonal antibody (STJ11103569) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from rat heart, using Citrate synthetase Rabbit monoclonal antibody (STJ11103569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Citrate synthetase antibody (367-466) [S9MR] (STJ11103569)

SKU:
STJ11103569

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Citrate synthetase (367-466) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CS
Gene ID: 1431
Uniprot ID: CISY_HUMAN
Immunogen Region: 367-466
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 367-466 of human Citrate synthetase (O75390).
Immunogen Sequence: HLPNDPMFKLVAQLYKIVPN VLLEQGKAKNPWPNVDAHSG VLLQYYGMTEMNYYTVLFGV SRALGVLAQLIWSRALGFPL ERPKSMSTEGLMKFVDSKSG
Post Translational Modifications Methylated. Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity.
Protein Name Citrate Synthase - Mitochondrial
Citrate
Si-Synthase
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Citrate Synthase - Mitochondrial antibody
Anti-Citrate antibody
Anti-Si-Synthase antibody
Anti-CS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance