| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Mouse/Rat/Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-CHMP2A (N-Term) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB. A dilution of 1:1000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | CHMP2A |
| Gene ID: | 27243 |
| Uniprot ID: | CHM2A_HUMAN |
| Immunogen Region: | N-Term |
| Specificity: | Specific for CHMP2A. |
| Immunogen: | A synthetic peptide from the n-terminal of human CHMP2A conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Function | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. (Microbial infection) The ESCRT machinery functions in topologically equivalent membrane fission events, such as the budding of enveloped viruses (HIV-1 and other lentiviruses). Involved in HIV-1 p6- and p9-dependent virus release. |
| Protein Name | Charged Multivesicular Body Protein 2a Chromatin-Modifying Protein 2a Chmp2a Putative Breast Adenocarcinoma Marker Bc-2 Vacuolar Protein Sorting-Associated Protein 2-1 Vps2-1 Hvps2-1 |
| Database Links | Reactome: R-HSA-162588 Reactome: R-HSA-1632852 Reactome: R-HSA-432720 Reactome: R-HSA-5620971 Reactome: R-HSA-917729 Reactome: R-HSA-9610379 Reactome: R-HSA-9615710 Reactome: R-HSA-9668328 Reactome: R-HSA-9679504 Reactome: R-HSA-9694676 |
| Cellular Localisation | Late Endosome Membrane Peripheral Membrane Protein Cytoplasmic Side Nucleus Envelope Localizes To The Midbody Of Dividing Cells Localized In Two Distinct Rings On Either Side Of The Fleming Body Localizes To The Reforming Nuclear Envelope On Chromatin Disks During Late Anaphase |
| Alternative Antibody Names | Anti-Charged Multivesicular Body Protein 2a antibody Anti-Chromatin-Modifying Protein 2a antibody Anti-Chmp2a antibody Anti-Putative Breast Adenocarcinoma Marker Bc-2 antibody Anti-Vacuolar Protein Sorting-Associated Protein 2-1 antibody Anti-Vps2-1 antibody Anti-Hvps2-1 antibody Anti-CHMP2A antibody Anti-BC2 antibody Anti-CHMP2 antibody |
Information sourced from Uniprot.org