• Western blot analysis of extracts of mouse testis, using CHIC2 Antibody (STJ110278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using CHIC2 antibody (STJ110278) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human stomach using CHIC2 antibody (STJ110278) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat brain using CHIC2 antibody (STJ110278) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using CHIC2 antibody (STJ110278) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human stomach using CHIC2 antibody (STJ110278) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using CHIC2 antibody (STJ110278) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of mouse testis, using CHIC2 Antibody (STJ110278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CHIC2 antibody (1-165) (STJ110278)

SKU:
STJ110278

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHIC2 (1-165) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHIC2
Gene ID: 26511
Uniprot ID: CHIC2_HUMAN
Immunogen Region: 1-165
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human CHIC2 (NP_036242.1).
Immunogen Sequence: MADFDEIYEEEEDEERALEE QLLKYSPDPVVVRGSGHVTV FGLSNKFESEFPSSLTGKVA PEEFKASINRVNSCLKKNLP VNVRWLLCGCLCCCCTLGCS MWPVICLSKRTRRSIEKLLE WENNRLYHKLCLHWRLSKRK CETNNMMEYVILIEFLPKTP IFRPD
Post Translational Modifications Palmitoylation in the CHIC motif is required for membrane association.
Protein Name Cysteine-Rich Hydrophobic Domain-Containing Protein 2
Brx-Like Translocated In Leukemia
Cellular Localisation Cell Membrane
Cytoplasmic Vesicle
Also Present At A Golgi-Like Vesicular Compartment And At Scattered Vesicles
Alternative Antibody Names Anti-Cysteine-Rich Hydrophobic Domain-Containing Protein 2 antibody
Anti-Brx-Like Translocated In Leukemia antibody
Anti-CHIC2 antibody
Anti-BTL antibody

Information sourced from Uniprot.org

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