• Immunofluorescence analysis of paraffin-embedded mouse testis using CFAP61 Rabbit polyclonal antibody (STJ11100153) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CFAP61 in paraffin-embedded Human colon using CFAP61 Rabbit polyclonal antibody (STJ11100153) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse eye, using CFAP61 Rabbit polyclonal antibody (STJ11100153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CFAP61 antibody (1-100) (STJ11100153)

SKU:
STJ11100153

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CFAP61 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFAP61
Gene ID: 26074
Uniprot ID: CFA61_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CFAP61 (NP_056400.3).
Immunogen Sequence: MSVLTSPRGKVEVVHCRRTE SQDVYCIKSLIRKFTCKLFG KLNIIYLLEKANLAVTLCND KEEIMAQATFLDYPNWNVAK QDDWVSVFRELDSDIPCTPL
Function May regulate cilium motility through its role in the assembly of the axonemal radial spokes.
Protein Name Cilia- And Flagella-Associated Protein 61
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Axoneme
Alternative Antibody Names Anti-Cilia- And Flagella-Associated Protein 61 antibody
Anti-CFAP61 antibody
Anti-C20orf26 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance