| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CEP63 (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CEP63 |
| Gene ID: |
80254 |
| Uniprot ID: |
CEP63_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CEP63 (NP_001035859.1). |
| Immunogen Sequence: |
MEALLEGIQNRGHGGGFLTS CEAELQELMKQIDIMVAHKK SEWEGRTHALETCLKIREQE LKSLRSQLDVTHKEVGMLHQ QVEEHEKIKQEMTMEYKQEL KKLHEELCILKRSYEKLQKK QMREFRGNTKNHREDRSEIE RLTAKIEEFRQKSLDWEKQR LIYQQQVSSLEAQRKALAEQ SEIIQAQLVNRKQKLESVEL SSQSEIQHLSSKLERANDTI CANELEIERLTMRVNDLVG |
| Post Translational Modifications | Polyubiquitinated via 'Lys-48'-linked ubiquitin, leading to its degradation. Deubiquitinated by USP36, promoting its stabilization. |
| Function | Required for normal spindle assembly. Plays a key role in mother-centriole-dependent centriole duplication.the function seems also to involve CEP152, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication. Reported to be required for centrosomal recruitment of CEP152.however, this function has been questioned. Also recruits CDK1 to centrosomes. Plays a role in DNA damage response. Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes.this leads to the inactivation of spindle assembly and delay in mitotic progression. Promotes stabilization of FXR1 protein by inhibiting FXR1 ubiquitination. |
| Protein Name | Centrosomal Protein Of 63 KdaCep63 |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCentriolar SatelliteColocalizes With Cdk5rap2Cep152 And Wdr62 In A Discrete Ring Around The Proximal End Of The Parental CentrioleAt This SiteA Cohesive Structure Is Predicted To Engage Parental Centrioles And Procentrioles |
| Alternative Antibody Names | Anti-Centrosomal Protein Of 63 Kda antibodyAnti-Cep63 antibodyAnti-CEP63 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
