| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CENPJ (1069-1338) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CENPJ |
| Gene ID: |
55835 |
| Uniprot ID: |
CENPJ_HUMAN |
| Immunogen Region: |
1069-1338 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1069-1338 of human CENPJ (NP_060921.3). |
| Immunogen Sequence: |
DKLANTSVRFQNSQISSGTQ VEKYKKNYLPMQGNPPRRSK SAPPRDLGNLDKGQAASPRE PLEPLNFPDPEYKEEEEDQD IQGEISHPDGKVEKVYKNGC RVILFPNGTRKEVSADGKTI TVTFFNGDVKQVMPDQRVIY YYAAAQTTHTTYPEGLEVLH FSSGQIEKHYPDGRKEITFP DQTVKNLFPDGQEESIFPDG TIVRVQRDGNKLIEFNNGQR ELHTAQFKRREYPDGTVKT |
| Post Translational Modifications | Phosphorylation at Ser-589 and Ser-595 by PLK2 is required for procentriole formation and centriole elongation. Phosphorylation by PLK2 oscillates during the cell cycle: it increases at G1/S transition and decreases during the exit from mitosis. Phosphorylation at Ser-595 is also mediated by PLK4 but is not a critical step in PLK4 function in procentriole assembly. |
| Function | Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles. Required for centriole elongation and for STIL-mediated centriole amplification. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner. May be involved in the control of centriolar-microtubule growth by acting as a regulator of tubulin release. |
| Protein Name | Centromere Protein JCenp-JCentrosomal P4.1-Associated ProteinLag-3-Associated ProteinLyst-Interacting Protein 1 |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-6804115Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleLocalized Within The Center Of Microtubule AstersDuring Centriole BiogenesisIt Is Concentrated Within The Proximal Lumen Of Both Parental Centrioles And Procentrioles |
| Alternative Antibody Names | Anti-Centromere Protein J antibodyAnti-Cenp-J antibodyAnti-Centrosomal P4.1-Associated Protein antibodyAnti-Lag-3-Associated Protein antibodyAnti-Lyst-Interacting Protein 1 antibodyAnti-CENPJ antibodyAnti-CPAP antibodyAnti-LAP antibodyAnti-LIP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
