• Western blot analysis of extracts of MCF7 cells, using CEACAM5 antibody (STJ11100101) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using CEACAM5 mouse monoclonal antibody (STJ11100101) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using CEACAM5 mouse monoclonal antibody (STJ11100101) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using CEACAM5 mouse monoclonal antibody (STJ11100101) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human hepatocellular carcinoma using CEACAM5 mouse monoclonal antibody (STJ11100101) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using CEACAM5 mouse monoclonal antibody (STJ11100101) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human urothelial carcinoma using CEACAM5 mouse monoclonal antibody (STJ11100101) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CEACAM5 antibody (35-685) [S1MM] (STJ11100101)

SKU:
STJ11100101

£212.50 - £501.50
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Host: Mouse
Applications: WB/IHC/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-CEACAM5 (35-685) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S1MM
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CEACAM5
Gene ID: 1048
Uniprot ID: CEAM5_HUMAN
Immunogen Region: 35-685
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-685 of human CEACAM5.
Immunogen Sequence: KLTIESTPFNVAEGKEVLLL VHNLPQHLFGYSWYKGERVD GNRQIIGYVIGTQQATPGPA YSGREIIYPNASLLIQNIIQ NDTGFYTLHVIKSDLVNEEA TGQFRVYPELPKPSISSNNS KPVEDKDAVAFTCEPETQDA TYLWWVNNQSLPVSPRLQLS NGNRTLTLFNVTRNDTASYK CETQNPVSARRSDSVILNVL YGPDAPTISPLNTSYRSGEN LNLSCHAASNPPAQYSWFV
Tissue Specificity Expressed in columnar epithelial and goblet cells of the colon (at protein level). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Post Translational Modifications Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
Function Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression.induces resistance to anoikis of colorectal carcinoma cells. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
Protein Name Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5
Carcinoembryonic Antigen
Cea
Meconium Antigen 100
Cd Antigen Cd66e
Database Links Reactome: R-HSA-163125
Reactome: R-HSA-202733
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Apical Cell Membrane
Cell Surface
Localized To The Apical Glycocalyx Surface
Alternative Antibody Names Anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 antibody
Anti-Carcinoembryonic Antigen antibody
Anti-Cea antibody
Anti-Meconium Antigen 100 antibody
Anti-Cd Antigen Cd66e antibody
Anti-CEACAM5 antibody
Anti-CEA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance