| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CDYL (245-544) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CDYL |
| Gene ID: |
9425 |
| Uniprot ID: |
CDYL_HUMAN |
| Immunogen Region: |
245-544 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 245-544 of human CDYL (NP_004815.3). |
| Immunogen Sequence: |
TNIQTSVTGVTASKRKFIDD RRDQPFDKRLRFSVRQTESA YRYRDIVVRKQDGFTHILLS TKSSENNSLNPEVMREVQSA LSTAAADDSKLVLLSAVGSV FCCGLDFIYFIRRLTDDRKR ESTKMAEAIRNFVNTFIQFK KPIIVAVNGPAIGLGASILP LCDVVWANEKAWFQTPYTTF GQSPDGCSTVMFPKIMGGAS ANEMLLSGRKLTAQEACGKG LVSQVFWPGTFTQEVMVRI |
| Tissue Specificity | Expressed in the hippocampus with reduced expression in epileptic tissue compared to normal adjacent tissue (at protein level). Ubiquitous. Expressed at moderate levels in all tissues examined. Isoform 2: Most abundantly expressed isoform. |
| Function | Isoform 2: Chromatin reader protein that recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively). Part of multimeric repressive chromatin complexes, where it is required for transmission and restoration of repressive histone marks, thereby preserving the epigenetic landscape. Required for chromatin targeting and maximal enzymatic activity of Polycomb repressive complex 2 (PRC2).acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes. Acts as a corepressor for REST by facilitating histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression. Involved in X chromosome inactivation in females: recruited to Xist RNA-coated X chromosome and facilitates propagation of H3K9me2 by anchoring EHMT2. Promotes EZH2 accumulation and H3K27me3 methylation at DNA double strand breaks (DSBs), thereby facilitating transcriptional repression at sites of DNA damage and homology-directed repair of DSBs. Required for neuronal migration during brain development by repressing expression of RHOA. By repressing the expression of SCN8A, contributes to the inhibition of intrinsic neuronal excitability and epileptogenesis. In addition to acting as a chromatin reader, acts as a hydro-lyase. Shows crotonyl-coA hydratase activity by mediating the conversion of crotonyl-CoA ((2E)-butenoyl-CoA) to beta-hydroxybutyryl-CoA (3-hydroxybutanoyl-CoA), thereby acting as a negative regulator of histone crotonylation. Histone crotonylation is required during spermatogenesis.down-regulation of histone crotonylation by CDYL regulates the reactivation of sex chromosome-linked genes in round spermatids and histone replacement in elongating spermatids. By regulating histone crotonylation and trimethylation of H3K27, may be involved in stress-induced depression-like behaviors, possibly by regulating VGF expression. Isoform 1: Not able to recognize and bind histone H3K9me3, histone H3K27me2 and histone H3K27me3, due to the presence of a N-terminal extension that inactivates the chromo domain. Isoform 3: Not able to recognize and bind histone H3K9me3, histone H3K27me2 and histone H3K27me3, due to the absence of the chromo domain. Acts as a negative regulator of isoform 2 by displacing isoform 2 from chromatin. |
| Protein Name | Chromodomain Y-Like ProteinCdy-LikeCrotonyl-Coa Hydratase |
| Cellular Localisation | Isoform 2: NucleusChromosomeRecognizes And Binds Histone H3 Trimethylated At 'Lys-9'Dimethylated At 'Lys-27' And Trimethylated At 'Lys-27' (H3k9me3H3k27me2 And H3k27me3Respectively) On ChromatinMultimerization Is Required For Chromatin-BindingRecruited To Sites Of Dna Double Strand Breaks In A Parp1-Dependent Fashion |
| Alternative Antibody Names | Anti-Chromodomain Y-Like Protein antibodyAnti-Cdy-Like antibodyAnti-Crotonyl-Coa Hydratase antibodyAnti-CDYL antibodyAnti-CDYL1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
