• Western blot analysis of extracts of various cell lines, using CDCA2 antibody (STJ113630) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using CDCA2 Rabbit polyclonal antibody (STJ113630) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDCA2 antibody (724-1023) (STJ113630)

SKU:
STJ113630

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDCA2 (724-1023) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDCA2
Gene ID: 157313
Uniprot ID: CDCA2_HUMAN
Immunogen Region: 724-1023
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 724-1023 of human CDCA2 (NP_689775.2).
Immunogen Sequence: SDSPKPALTLQQGQEFSAGG QNAENLCQFFKISPDLNIKC ERKDDFLGAAEGKLQCNRLM PNSQKDCHCLGDVLIENTKE SKSQSEDLGRKPMESSSVVS CRDRKDRRRSMCYSDGRSLH LEKNGNHTPSSSVGSSVEIS LENSELFKDLSDAIEQTFQR RNSETKVRRSTRLQKDLENE GLVWISLPLPSTSQKAKRRT ICTFDSSGFESMSPIKETVS SRQKPQMAPPVSDPENSQG
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated by CDK1. May regulate its subcellular location.
Function Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.
Protein Name Cell Division Cycle-Associated Protein 2
Recruits Pp1 Onto Mitotic Chromatin At Anaphase Protein
Repo-Man
Cellular Localisation Nucleus
Excluded From The Nucleolus
Present In Nucleoplasm Throughout The G1
S And G2 Stages Of The Cell Cycle
During M Phase
It Becomes Diffuse Throughout The Cell As The Nuclear Membrane Breaks Down
And Faintly Accumulates Later On Metaphase Chromatin
As The Cell Progresses To Anaphase
It Accumulates On Chromatin
Alternative Antibody Names Anti-Cell Division Cycle-Associated Protein 2 antibody
Anti-Recruits Pp1 Onto Mitotic Chromatin At Anaphase Protein antibody
Anti-Repo-Man antibody
Anti-CDCA2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance