Host: |
Rabbit |
Applications: |
WB/IHC |
Reactivity: |
Human |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Rabbit monoclonal antibody anti-Cdc25B (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications. |
Clonality: |
Monoclonal |
Clone ID: |
S2MR |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
Purification: |
Affinity purification |
Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: |
CDC25B |
Gene ID: |
994 |
Uniprot ID: |
MPIP2_HUMAN |
Immunogen Region: |
100-200 |
Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CDC25B (P30305). |
Immunogen Sequence: |
SSESSESSDAGLCMDSPSPM DPHMAEQTFEQAIQAASRII RNEQFAIRRFQSMPVRLLGH SPVLRNITNSQAPDGRRKSE AGSGAASSSGEDKENDGFVF K |
Post Translational Modifications | Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. |
Function | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. |
Protein Name | M-Phase Inducer Phosphatase 2Dual Specificity Phosphatase Cdc25b |
Database Links | Reactome: R-HSA-69273Reactome: R-HSA-69656Reactome: R-HSA-8862803 |
Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle Pole |
Alternative Antibody Names | Anti-M-Phase Inducer Phosphatase 2 antibodyAnti-Dual Specificity Phosphatase Cdc25b antibodyAnti-CDC25B antibodyAnti-CDC25HU2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance