| Host: |
Rat |
| Applications: |
FC/IF/IHC |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rat monoclonal antibody anti-CD8a is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
IBL-3/25 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgGk |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
Flow cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate antigen retrieval method for staining of formalin-fixed tissues has not been established to date |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
Cd8a |
| Gene ID: |
12525 |
| Uniprot ID: |
CD8A_MOUSE |
| Immunogen: |
A Wistar rat was immunized with mouse lymph node cells. Fusion partner: SP-2/0. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. |
| Protein Name | T-Cell Surface Glycoprotein Cd8 Alpha ChainT-Cell Surface Glycoprotein Lyt-2Cd Antigen Cd8a |
| Database Links | Reactome: R-MMU-198933 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCd8a Localizes To Lipid Rafts Only When Associated With Its Partner Cd8b |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd8 Alpha Chain antibodyAnti-T-Cell Surface Glycoprotein Lyt-2 antibodyAnti-Cd Antigen Cd8a antibodyAnti-Cd8a antibodyAnti-Lyt-2 antibodyAnti-Lyt2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
