| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD86 |
| Gene ID: | 942 |
| Uniprot ID: | CD86_HUMAN |
| Immunogen Region: | 24-247 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-247 of human CD86 (NP_787058.4). |
| Immunogen Sequence: | APLKIQAYFNETADLPCQFA NSQNQSLSELVVFWQDQENL VLNEVYLGKEKFDSVHSKYM GRTSFDSDSWTLRLHNLQIK DKGLYQCIIHHKKPTGMIRI HQMNSELSVLANFSQPEIVP ISNITENVYINLTCSSIHGY PEPKKMSVLLRTKNSTIEYD GIMQKSQDNVTELYDVSISL SVSFPDVTSNMTIFCILETD KTRLLSSPFSIELEDPQPPP DHIP |
| Tissue Specificity | Expressed by activated B-lymphocytes and monocytes. |
| Post Translational Modifications | Polyubiquitinated.which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. |
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. Isoform 2: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B. |
| Protein Name | T-Lymphocyte Activation Antigen Cd86 Activation B7-2 Antigen B70 Bu63 Ctla-4 Counter-Receptor B7.2 Fun-1 Cd Antigen Cd86 |
| Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-2219530 Reactome: R-HSA-389356 Reactome: R-HSA-389357 Reactome: R-HSA-389359 Reactome: R-HSA-389513 Reactome: R-HSA-6783783 Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Lymphocyte Activation Antigen Cd86 antibody Anti-Activation B7-2 Antigen antibody Anti-B70 antibody Anti-Bu63 antibody Anti-Ctla-4 Counter-Receptor B7.2 antibody Anti-Fun-1 antibody Anti-Cd Antigen Cd86 antibody Anti-CD86 antibody Anti-CD28LG2 antibody |
Information sourced from Uniprot.org