• Western blot analysis of extracts of RAW264. 7 cells, using CD68 antibody (STJ11103378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of Raji cells, using CD68 antibody (STJ11103378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of RAW 264 7 and Jurkat (Negative sample) cells using CD68 rabbit polyclonal antibody (STJ11103378) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using CD68 rabbit polyclonal antibody (STJ11103378) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD68 antibody (50-150) (STJ11103378)

SKU:
STJ11103378

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD68 (50-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Cd68
Gene ID: 12514
Uniprot ID: CD68_MOUSE
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of mouse CD68 (NP_001277987.1).
Immunogen Sequence: SHRPTTTSHGNVTVHTSSGP TTVTHNPATTTSHGNATISH ATVSPTTNGTATSPRSSTVG PHPGPPPPSPSPRSKGALGN YTWANGSQPCVQLQAQIQIR I
Tissue Specificity Expressed in tissue macrophages and to a lesser extent in dendritic cells.
Post Translational Modifications N- and O-glycosylated.
Function Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Protein Name Macrosialin
Cd Antigen Cd68
Database Links Reactome: R-MMU-6798695
Cellular Localisation Isoform Long: Endosome Membrane
Single-Pass Type I Membrane Protein
Lysosome Membrane
Isoform Short: Cell Membrane
Alternative Antibody Names Anti-Macrosialin antibody
Anti-Cd Antigen Cd68 antibody
Anti-Cd68 antibody

Information sourced from Uniprot.org

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