• Western blot analysis of extracts of various cell lines, using CD5 antibody (STJ28962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CD5 rabbit polyclonal antibody (STJ28962) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD5 rabbit polyclonal antibody (STJ28962) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD5 antibody (158-371) (STJ28962)

SKU:
STJ28962

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD5 (158-371) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD5
Gene ID: 921
Uniprot ID: CD5_HUMAN
Immunogen Region: 158-371
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 158-371 of human CD5 (NP_055022.2).
Immunogen Sequence: RLQLVAQSGGQHCAGVVEFY SGSLGGTISYEAQDKTQDLE NFLCNNLQCGSFLKHLPETE AGRAQDPGEPREHQPLPIQW KIQNSSCTSLEHCFRKIKPQ KSGRVLALLCSGFQPKVQSR LVGGSSICEGTVEVRQGAQW AALCDSSSARSSLRWEEVCR EQQCGSVNSYRVLDAGDPTS RGLFCPHQKLSQCHELWERN SYCKKVFVTCQDPN
Post Translational Modifications Phosphorylated on tyrosine residues by LYN.this creates binding sites for PTPN6/SHP-1.
Function May act as a receptor in regulating T-cell proliferation.
Protein Name T-Cell Surface Glycoprotein Cd5
Lymphocyte Antigen T1/Leu-1
Cd Antigen Cd5
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd5 antibody
Anti-Lymphocyte Antigen T1/Leu-1 antibody
Anti-Cd Antigen Cd5 antibody
Anti-CD5 antibody
Anti-LEU1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance