• Flow cytometry: 1X10^6 HeLa cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 Mu l/Test, left) or CD44 Rabbit monoclonal antibody (STJ11101558, 2. 5 Mu g/mL, right).
  • Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and HeLa cells (right) were surface-stained with CD44 Rabbit monoclonal antibody (STJ11101558, 2. 5 Mu g/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 Mu l/Test, blue line) , followed by Alexa Fluor® 647 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).
  • Immunofluorescence analysis of HeLa cells using CD44 Rabbit monoclonal antibody (STJ11101558) at dilution of 1:1000 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CD44 in paraffin-embedded human skin using CD44 Rabbit monoclonal antibody (STJ11101558) at dilution of 1:2000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CD44 in paraffin-embedded human liver cancer using CD44 Rabbit monoclonal antibody (STJ11101558) at dilution of 1:2000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using CD44 Rabbit monoclonal antibody (STJ11101558) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10S.

Anti-CD44 antibody (20-178) [S8MR] (STJ11101558)

SKU:
STJ11101558

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Host: Rabbit
Applications: WB/IHC/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD44 (20-178) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD44
Gene ID: 960
Uniprot ID: CD44_HUMAN
Immunogen Region: 20-178
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-178 of human CD44 (NP_000601.3).
Immunogen Sequence: AQIDLNITCRFAGVFHVEKN GRYSISRTEAADLCKAFNST LPTMAQMEKALSIGFETCRY GFIEGHVVIPRIHPNSICAA NNTGVYILTSNTSQYDTYCF NASAPPEEDCTSVTDLPNAF DGPITITIVNRDGTRYVQKG EYRTNPEDIYPSNPTDDDV
Tissue Specificity Detected in fibroblasts and urine (at protein level). Detected in placenta (at protein level). Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Post Translational Modifications Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated.contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated.activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
Function Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion.
Protein Name Cd44 Antigen
Cdw44
Epican
Extracellular Matrix Receptor Iii
Ecmr-Iii
Gp90 Lymphocyte Homing/Adhesion Receptor
Hutch-I
Heparan Sulfate Proteoglycan
Hermes Antigen
Hyaluronate Receptor
Phagocytic Glycoprotein 1
Pgp-1
Phagocytic Glycoprotein I
Pgp-I
Cd Antigen Cd44
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-2160916
Reactome: R-HSA-6798695
Reactome: R-HSA-877300
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Microvillus
Secreted
Colocalizes With Actin In Membrane Protrusions At Wounding Edges
Co-Localizes With Rdx
Ezr And Msn In Microvilli
Localizes To Cholesterol-Rich Membrane-Bound Lipid Raft Domains
Alternative Antibody Names Anti-Cd44 Antigen antibody
Anti-Cdw44 antibody
Anti-Epican antibody
Anti-Extracellular Matrix Receptor Iii antibody
Anti-Ecmr-Iii antibody
Anti-Gp90 Lymphocyte Homing/Adhesion Receptor antibody
Anti-Hutch-I antibody
Anti-Heparan Sulfate Proteoglycan antibody
Anti-Hermes Antigen antibody
Anti-Hyaluronate Receptor antibody
Anti-Phagocytic Glycoprotein 1 antibody
Anti-Pgp-1 antibody
Anti-Phagocytic Glycoprotein I antibody
Anti-Pgp-I antibody
Anti-Cd Antigen Cd44 antibody
Anti-CD44 antibody
Anti-LHR antibody
Anti-MDU2 antibody
Anti-MDU3 antibody
Anti-MIC4 antibody

Information sourced from Uniprot.org

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