• Immunofluorescence analysis of THP-1 cells using CD4 Rabbit polyclonal antibody (STJ11105070) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse spleen cells using CD4 Rabbit polyclonal antibody (STJ11105070) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of Mouse thymus, using CD4 Rabbit polyclonal antibody (STJ11105070) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD4 antibody (STJ11105070)

SKU:
STJ11105070

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD4 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Cd4
Gene ID: 12504
Uniprot ID: CD4_MOUSE
Immunogen: Recombinant protein of mouse CD4.
Tissue Specificity Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.
Post Translational Modifications Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation plays an important role for CD4 internalization.
Function Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
Protein Name T-Cell Surface Glycoprotein Cd4
T-Cell Differentiation Antigen L3t4
T-Cell Surface Antigen T4/Leu-3
Cd Antigen Cd4
Database Links Reactome: R-MMU-1462054
Reactome: -MMU-202424
Reactome: -MMU-202427
Reactome: -MMU-202430
Reactome: -MMU-202433
Reactome: -MMU-389948
Reactome: -MMU-449836
Reactome: -MMU-8856825
Reactome: -MMU-8856828
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd4 antibody
Anti-T-Cell Differentiation Antigen L3t4 antibody
Anti-T-Cell Surface Antigen T4/Leu-3 antibody
Anti-Cd Antigen Cd4 antibody
Anti-Cd4 antibody

Information sourced from Uniprot.org

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