| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CD3G (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 23kDa band observed in lysates of cell line Jurkat (calculated MW of 20.5kDa according to NP_000064.1). Recommended concentration: 0.3-1µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CD3G |
| Gene ID: | 917 |
| Uniprot ID: | CD3G_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000064.1 |
| Immunogen Sequence: | QDGVRQSRASDKQT |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G. |
| Protein Name | T-Cell Surface Glycoprotein Cd3 Gamma Chain T-Cell Receptor T3 Gamma Chain Cd Antigen Cd3g |
| Database Links | Reactome: R-HSA-198933 Reactome: R-HSA-202424 Reactome: R-HSA-202427 Reactome: R-HSA-202430 Reactome: R-HSA-202433 Reactome: R-HSA-2029481 Reactome: R-HSA-2029482 Reactome: R-HSA-2029485 Reactome: R-HSA-389948 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 Reactome: R-HSA-9664323 Reactome: R-HSA-9664422 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd3 Gamma Chain antibody Anti-T-Cell Receptor T3 Gamma Chain antibody Anti-Cd Antigen Cd3g antibody Anti-CD3G antibody Anti-T3G antibody |
Information sourced from Uniprot.org