• Immunofluorescence analysis of Jurkat cells using Human CD3E Rabbit polyclonal antibody (STJ11105519) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Human CD3E Rabbit polyclonal antibody (STJ11105519) at 1:900 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Negative control (NC) : PC-3. Exposuretime: 1s.

Anti-CD3E antibody (22-126) (STJ11105519)

SKU:
STJ11105519

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-T-Cell Surface Glycoprotein Cd3 Epsilon Chain (22-126) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD3E
Gene ID: 916
Uniprot ID: CD3E_HUMAN
Immunogen Region: 22-126
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-126 of human CD3E (NP_000724.1).
Immunogen Sequence: DGNEEMGGITQTPYKVSISG TTVILTCPQYPGSEILWQHN DKNIGGDEDDKNIGSDEDHL SLKEFSELEQSGYYVCYPRG SKPEDANFYLYLRARVCENC MEMD
Post Translational Modifications Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.
Protein Name T-Cell Surface Glycoprotein Cd3 Epsilon Chain
T-Cell Surface Antigen T3/Leu-4 Epsilon Chain
Cd Antigen Cd3e
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-389948
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd3 Epsilon Chain antibody
Anti-T-Cell Surface Antigen T3/Leu-4 Epsilon Chain antibody
Anti-Cd Antigen Cd3e antibody
Anti-CD3E antibody
Anti-T3E antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance