| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CD31/PECAM1 (611-678) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Pecam1 |
| Gene ID: |
29583 |
| Uniprot ID: |
PECA1_RAT |
| Immunogen Region: |
611-678 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 611-678 of rat CD31/PECAM1 (NP_113779.1). |
| Immunogen Sequence: |
YFLRKAKAKQKPVEMSRPAV PLLNSNSEKVSEPSVETNSH YDSQNMDVEYTEVEVSSLEP HQENGRLP |
| Post Translational Modifications | Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. |
| Function | Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells.PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis. |
| Protein Name | Platelet Endothelial Cell Adhesion MoleculePecam-1Cd Antigen Cd31 |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-202733Reactome: -RNO-210990Reactome: -RNO-216083Reactome: -RNO-432142Reactome: -RNO-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane RaftCell JunctionLocalizes To The Lateral Border Recycling Compartment (Lbrc) And Recycles From The Lbrc To The Junction In Resting Endothelial CellsCell Surface Expression On Neutrophils Is Down-Regulated Upon Fmlp Or Cxcl8/Il8-Mediated Stimulation |
| Alternative Antibody Names | Anti-Platelet Endothelial Cell Adhesion Molecule antibodyAnti-Pecam-1 antibodyAnti-Cd Antigen Cd31 antibodyAnti-Pecam1 antibodyAnti-Pecam antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![C57BL/6 murine splenocytes were stained with Anti-CD31 antibody (STJA0001006) [Used at 0.05 µg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/145859/292516/STJA0001006_1__80034.1631206382.jpg?c=1 "C57BL/6 murine splenocytes were stained with Anti-CD31 antibody (STJA0001006) [Used at 0.05 µg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.")
