• Western blot analysis of extracts of various cell lines, using CD31/PECAM1 antibody (STJ11103330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD31/PECAM1 antibody (611-678) (STJ11103330)

SKU:
STJ11103330

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD31/PECAM1 (611-678) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Pecam1
Gene ID: 29583
Uniprot ID: PECA1_RAT
Immunogen Region: 611-678
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 611-678 of rat CD31/PECAM1 (NP_113779.1).
Immunogen Sequence: YFLRKAKAKQKPVEMSRPAV PLLNSNSEKVSEPSVETNSH YDSQNMDVEYTEVEVSSLEP HQENGRLP
Post Translational Modifications Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
Function Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells.PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.
Protein Name Platelet Endothelial Cell Adhesion Molecule
Pecam-1
Cd Antigen Cd31
Database Links Reactome: R-RNO-114608
Reactome: -RNO-202733
Reactome: -RNO-210990
Reactome: -RNO-216083
Reactome: -RNO-432142
Reactome: -RNO-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Cell Junction
Localizes To The Lateral Border Recycling Compartment (Lbrc) And Recycles From The Lbrc To The Junction In Resting Endothelial Cells
Cell Surface Expression On Neutrophils Is Down-Regulated Upon Fmlp Or Cxcl8/Il8-Mediated Stimulation
Alternative Antibody Names Anti-Platelet Endothelial Cell Adhesion Molecule antibody
Anti-Pecam-1 antibody
Anti-Cd Antigen Cd31 antibody
Anti-Pecam1 antibody
Anti-Pecam antibody

Information sourced from Uniprot.org

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