• Flow cytometric analysis of CD28 Rabbit monoclonal antibody Cocotail (10 Mu g/mL) in Jurkat cells (Orange) compare to Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) Isotype control (Blue) and non-staining control (Red).
  • Western blot analysis of extracts of various cell lines, using CD28 antibody (STJ11103275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD28 antibody [S5MR] (STJ11103275)

SKU:
STJ11103275

£146.50 - £639.50
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Host: Rabbit
Applications: WB/FC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD28 is suitable for use in Western Blot and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
FC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD28
Gene ID: 940
Uniprot ID: CD28_HUMAN
Immunogen: Recombinant protein of human CD28 Full length ( P10747 ).
Immunogen Sequence: MLRLLLALNLFPSIQVTGNK ILVKQSPMLVAYDNAVNLSC KYSYNLFSREFRASLHKGLD SAVEVCVVYGNYSQQLQVYS KTGFNCDGKLGNESVTFYLQ NLYVNQTDIYFCKIEVMYPP PYLDNEKSNGTIIHVKGKHL CPSPLFPGPSKPFWVLVVVG GVLACYSLLVTVAFIIFWVR SKRSRLLHSDYMNMTPRRPG PTRKHYQPYAPPRDFAAYRS
Tissue Specificity Expressed in T-cells and plasma cells, but not in less mature B-cells.
Post Translational Modifications CD40LG induces tyrosine phosphorylation of isoform 3.
Function Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation. Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells.
Protein Name T-Cell-Specific Surface Glycoprotein Cd28
Tp44
Cd Antigen Cd28
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-164939
Reactome: R-HSA-2219530
Reactome: R-HSA-389356
Reactome: R-HSA-389357
Reactome: R-HSA-389359
Reactome: R-HSA-6811558
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Isoform 3: Cell Surface
Alternative Antibody Names Anti-T-Cell-Specific Surface Glycoprotein Cd28 antibody
Anti-Tp44 antibody
Anti-Cd Antigen Cd28 antibody
Anti-CD28 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance