• Western blot analysis of various lysates using CD276/B7-H3 Rabbit polyclonal antibody (STJ11104467) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD276 antibody (60-159) (STJ11104467)

SKU:
STJ11104467

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cd276 antigen (60-159) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD276
Gene ID: 80381
Uniprot ID: CD276_HUMAN
Immunogen Region: 60-159
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 60-159 of human CD276/B7-H3 (NP_001019907.1).
Immunogen Sequence: LAQLNLIWQLTDTKQLVHSF AEGQDQGSAYANRTALFPDL LAQGNASLRLQRVRVADEGS FTCFVSIRDFGSAAVSLQVA APYSKPSMTLEPNKDLRPGD
Tissue Specificity Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
Function May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Protein Name Cd276 Antigen
4ig-B7-H3
B7 Homolog 3
B7-H3
Costimulatory Molecule
Cd Antigen Cd276
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cd276 Antigen antibody
Anti-4ig-B7-H3 antibody
Anti-B7 Homolog 3 antibody
Anti-B7-H3 antibody
Anti-Costimulatory Molecule antibody
Anti-Cd Antigen Cd276 antibody
Anti-CD276 antibody
Anti-B7H3 antibody
Anti-PSEC0249 antibody
Anti-UNQ309 antibody
Anti-PRO352 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance